DNA shuffling

Using DNA shuffling application and prospect in plant improvement

DNA改组技术在植物改良中的利用前景

The Development and Application of DNA Shuffling

DNA改组技术发展与应用

There were many successful examples to use DNA shuffling to improve cellulase in foreign countries .

国外已有大量成功利用DNA改组技术对纤维素酶分子进行改良的的例子。

DNA shuffling performed well in the evolution of DNA , protein and organism .

无论DNA、蛋白质还是生物体的进化，DNA改组都有十分突出的表现。

SOE by PCR is one of DNA shuffling technologies for molecular evolution engineering .

交错延伸剪接PCR是一种用于分子进化研究的DNA改组技术。

Study on DNA Shuffling Method for Changing the Promoter Activity of aac C1 Gene

利用DNA改组技术改造aacC1基因启动子活性的研究

Phage display and DNA shuffling are important and efficient techniques for directed molecular evolution .

DNA重排和噬菌体展示及筛选是体外定向分子进化的重要核心技术。

Directed Evolution of High Activity β - Glucuronidase by DNA Shuffling and Screening

通过DNA改组技术获得高活性β-葡萄糖苷酸酶

DNA Shuffling and Its Application to Biological Pharmaceuticals

DNA改组（DNAshuffling）在生物药学中的应用

DNA Shuffling & technological revolution of directed evolution

DNA改组&定向进化的技术革命

DNA shuffling and in vitro molecular evolution

DNA重排及体外分子进化

Purification and characterization of recombinant phytase evolved by DNA shuffling in vitro

经DNA改组的植酸酶纯化和酶学性质

A facile DNA shuffling protocol was introduced .

介绍了一个简便的DNA改组操作程序。

Conclusion : The study lays a foundation to establish a screening platform for DNA shuffling based on yeast surface display system .

结论：本研究为建立基于酵母表面展示系统的DNA改组筛选平台奠定了基础。

Objective : To use DNA shuffling for directed evolution of HCV C and E1 with different genotypes .

目的：利用DNA改组技术进行不同亚型的HCVC和E1区的人工进化。

Objective To improve the bioactivity of antithrombin ⅲ( AT - ⅲ) significantly by DNA shuffling .

目的利用DNA改组技术大幅度提高抗凝血酶Ⅲ（AT-Ⅲ）的生物活性。

DNA Shuffling and Screening for Obtaining Lactose Super - Repressor in Escherichia Coli

DNA改组和筛选获得大肠杆菌乳糖操纵子超级阻遏蛋白

Affinity maturation of a single-chain antibody for hepatocellular carcinoma by error-prone PCR and DNA shuffling

错配PCR和DNA改组技术提高抗肝癌单链抗体亲和力

Recombinant phytase evolved by DNA shuffling was purified by ultra-filtration and DEAE-Sepharose F.

经DNA改组的重组植酸酶通过有机膜超滤、DEAE-SepharoseF。

In order to solve this problem , we use DNA Shuffling to restructure the Bt gene technology , and getting a batch of clones .

为了解决此问题，我们运用DNAshuffling技术把Bt基因进行改组，获得了一批克隆子。

This research adopted vitro directional evolution technology of DNA shuffling , we established EGI mutant library from BL21 ( DE3 ) .

采用DNAshuffling的体外定向进化技术，本研究建立了来源于BL21（DE3）的EGI突变体库。

DNA Shuffling developed rapidly and was applied widely in improving the functions of enzyme , optimisation of pharmaceutical proteins , amelioration of Vaccines and etc.

DNA改组技术发展迅速并广泛应用于酶的改良、药物蛋白优化、疫苗改进等领域，具有广阔应用发展前景。

DNA shuffling and genome shuffling methods can increase our understanding of metabolic networks and their molecular regulation mechanisms , integrating and complementing rational metabolic design strategies .

DNA改组及基因组改组技术可以加深对代谢网络及其分子调节机制的理解，是对合理代谢设计策略的完善和补充。

The construction strategy of mutagenesis protein library includes oligonucleotide-mediated site-directed mutagenesis , cassette mutagenesis , DNA shuffling , and error-prone PCR .

突变蛋白库构建策略包括：寡核苷酸介导的直接位点突变、盒式突变、DNAshuffling以及错倾PCR。

This review illustrates the applications of DNA shuffling and genome shuffling in metabolic engineering and prospects the perspective and methodology in metabolic engineering by genome shuffling .

本文首先分别介绍了DNA改组、基因组改组在代谢工程中的应用，进而展望了基于基因组改组的代谢工程方法步骤及发展方向。

In the past few years , many new protocols for directed evolution were developed on the basis of oligonucleotide - directed random mutation , error-prone PCR and DNA shuffling .

最近几年，在过去常用的寡核苷酸介导的随机突变、易错PCR和DNA改组等方法的基础上又出现了一些新的定向进化方法。

The α - amylase gene of Xanthomonas campestris pv Campestris 8004 was subjected to DNA shuffling in vitro for mutants with enhanced catalytic activity .

本研究通过对野油菜黄单胞菌α-淀粉酶基因进行两轮单基因的改组，筛选到了两株酶活力提高（分别为出发基因的2倍、3倍）的突变株。

In addition to new pathway construction , DNA shuffling and genome shuffling are applied to rapid optimization of known or unknown pathway , which greatly promotes the development and applications of metabolic engineering .

DNA改组及基因组改组可以构建新的代谢途径、对已知或未知的代谢途径进行快速优化，极大地促进了代谢工程的进一步发展和应用。

Objective : To display the envelope protein of dengue virus type 2 strain 43 ( D2-43 ) on yeast cell surface and explore a screening platform for DNA shuffling with yeast surface display system .

目的：在酵母细胞表面展示登革2型病毒43株（D243）的E基因，探索利用酵母表面展示系统建立DNA改组筛选平台的可行性。

Over the twenty years , it has branched into three characteristic strategies : splicing by overlapping extension ( SOE ), jumping polymerase chain reaction ( JPCR ) and DNA shuffling .

经过20多年的发展，该技术已形成三个各具特色的方法分支：重叠延伸拼接、跳跃PCR和DNA重排。